Anti-Hepatocyte Growth Factor (Hepatopoeitin-A, Scatter Factor, SF, HGF, HPTA)

Hepatocyte Growth Factor (HGF) is structurally similar, but not identical to scatter factor, a molecule shown to stimulate the dissociation and scattering of epithelial cells. It mapped to 7q21.1 and is involved in liver regeneration. Human HGF has been purified approximately 209,000-fold with 18% yield from plasma of a patient with fulminant hepatic failure. It consists of 2 chains, heavy and light with molecular weights of 54-65kD and 31.5-34.5kD, respectively. HGF is a potent mitogen, morphogen and motogen to both endothelial and epithelial cell types and is linked to a tyrosine kinase, proto-oncogene, c-met receptor. It indicated that HGF may serve as a paracrine mediator to control placental development and growth. Applications: Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 1ug/ml, Immunohistochemistry (Frozen): 1ug/ml, Immunohistochemistry (Paraffin): 1ug/ml. Heat mediated antigen retrival recommended., Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Crossreactivity by Application: Immunocytochemistry: human, Immunohistochemistry (Frozen): mouse and rat, Immunohistochemistry (Paraffin): human, mouse and rat, Western Blot: human and rat, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.