Immunogen:synthetic peptide. The epitope recognized by A302-822A maps to a region between residue 650 and 700 of human Holliday Junction Recognition Protein using the numbering given in entry NP_060880.3 (GeneID 55355).
Bemerkungen:Holliday junction recognition protein (HJURP) is a centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. HJURP acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. HJURP directly binds Holliday junctions. Alternative names for HJURP include 14-3-3-associated AKT substrate,fetal liver-expressing gene 1 protein, up-regulated in lung cancer 9, FAKTS, FLEG1, hFLEG1, and URLC9. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8NCD3]
Rabbit anti-HJURP Antibody, Affinity Purified
Detection of Human HJURP by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HJURP antibody A302-822A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. HJURP was also immunoprecipitated by rabbit anti-HJURP antibody A302-823A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of Human HJURP by Immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-HJURP (Cat. No. A302-822A) used at a dilution of 1:1,000 (1µg/mg) Detection: DAB staining using Immunohistochemistry Accessory Kit (Cat. No. IHC-101).
Gene and Protein Information
Zasadzi?ska E, Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition. EMBO J. 2013 Jul 31;32(15):2113-24. ICC
Jaramillo-Lambert A, Acidic Nucleoplasmic DNA-binding Protein (And-1) Controls Chromosome Congression by Regulating the Assembly of Centromere Protein A (CENP-A) at Centromeres. J Biol Chem. 2013 Jan 18;288(3):1480-8.