Anti-Axin2

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 complexes with APC, GSK3 beta, Dvl and beta-catenin and promotes the GSK3 beta-mediated phosphorylation and subsequent degradation of beta-catenin. Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling. In addition to its role in the Wnt signaling pathway, Axin1 complexes with MEKK1 and causes the activation of c-Jun amino-terminal kinase (JNK/SAPK). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice. Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, Immunoprecipitation: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.