Anti-6X HIS EPITOPE TAG, clone 33D10.D2

Item number Size Datasheet Manual SDS Delivery time Quantity Price
200-301-382S 25 µl - -

6 - 10 business days*

180.00€
 
This affinity purified antibody is directed against the 6X His motif and is useful in determining... more
Product information "Anti-6X HIS EPITOPE TAG, clone 33D10.D2"
This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.  To date, this antibody has reacted with all His tagged proteins so far tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.  The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Supplier: Rockland Immunochemicals
Supplier-Nr: 200-301-382S

Properties

Application: ELISA, FC, IHC, IP, WB
Antibody Type: Monoclonal
Clone: 33D10.D2
Conjugate: No
Host: Mouse
Immunogen: 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Format: Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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