Reaktivität:human, rat, bovine (expected: mouse, rabbit, guinea pig, swine, orangutan, rhesus monkey, gorilla, chimpanzee, white-tufted-ear marmoset, crab-eating macaque, african elephant, chinese hamster, naked mole rat, northern white-cheeked gibbon)
Immunogen:synthetic peptide. The epitope recognized by A300-508A maps to a region between residues 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-2 chain using the numbering given in entry AAH69823.1 (GeneID 5563).
Bemerkungen:AMP-activated protein kinase (AMPK) is a heterotrimeric protein made up of a catalytic alpha subunit, and a regulatory beta and gamma subunit. There are two distinct genes for the alpha subunit, alpha1 and alpha2. AMPK alpha 2 is the serine/threonine kinase catalytic subunit of the AMPK. AMPK is responsible for regulating fatty acid and cholesterol synthesis. AMPK is activated in response to a wide variety of metabolic stresses and switches off anabolic pathways when ATP levels are depleted. AMPK alpha 2 is also known as 5-AMP-activated protein kinase catalytic subunit alpha-2, AMPK alpha-2 chain, PRKAA, PRKAA2, AMPK, and AMPK2,
Detection of Human and Mouse AMPK alpha 2 by Western Blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse Renca, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-AMPK alpha 2 antibody A300-508A (lot A300-508A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Gene and Protein Information
Rabbit, Guinea pig_10141, Pig, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Little brown bat, Chinese hamster, Naked mole rat, Small-eared galago, Crab-eating macaque, White-tufted-ear marmoset, African elephant, Northern white-cheeked gibbon, Thirteen-lined ground squirrel less...
Hardman SE, effects of age and muscle contraction on AMPK activity and heterotrimer composition. Exp Gerontol. 2014 Apr 17. pii: S0531-5565(14)00128-4.
Lin D, Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: The role of AMP-activated protein kinase α2 subunit. Mol Nutr Food Res. 2014 May;58(5):1005-15.
Harmel E, AMPK in the small intestine in normal and pathophysiological conditions Endocrinology. 2014 Jan 1:en20131750. WB
Yu JW, Hepatitis C virus core protein induces hepatic metabolism disorders through down-regulation of the SIRT1-AMPKsignaling pathway. Int J Infect Dis. 2013 Jul;17(7):e539-45. IP
Scheele C et al., Satellite cells derived from obese humans with type 2 diabetes and differentiated into myocytes in vitro exhibit abnormal response to IL-6. PLoS One. 2012;7(6):e39657. IP
Wang S et al., AMPKalpha1 deletion shortens erythrocyte life span in mice: role of oxidative stress. J Biol Chem. 2010 Jun 25;285(26):19976-85. WB
Bendayan M et al., Association of AMP-activated protein kinase subunits with glycogen particles as revealed in situ by immunoelectron microscopy. J Histochem Cytochem. 2009 Oct;57(10):963-71. WB
Hou X et al., SIRT1 regulates hepatocyte lipid metabolism through activating AMP-activated protein kinase. J Biol Chem. 2008 Jul 18;283(29):20015-26. WB
Qin S et al., Implication of S-adenosylhomocysteine hydrolase in inhibition of TNF-alpha- and IL-1beta-induced expression of inflammatory mediators by AICAR in RPE cells. Invest Ophthalmol Vis Sci. 2008 Mar;49(3):1274-81. WB
Cammisotto PG et al., Control of glycogen synthase through ADIPOR1-AMPK pathway in renal distal tubules of normal and diabetic rats. Am J Physiol Renal Physiol. 2008 Apr;294(4):F881-9. WB, ICC
Qin S et al., alpha2 But not alpha1 AMP-activated protein kinase mediates oxidative stress-induced inhibition of retinal pigment epithelium cell phagocytosis of photoreceptor outer segments. J Biol Chem. 2008 Mar 14;283(11):6744-51. WB
Zang M et al., Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor-deficient mice. Diabetes. 2006 Aug;55(8):2180-91. WB
Doi M et al., Isoleucine, a blood glucose-lowering amino acid, increases glucose uptake in rat skeletal muscle in the absence of increases in AMP-activated protein kinase activity. J Nutr. 2005 Sep;135(9):2103-8. IP
Rubin LJ et al., Metabolic activation of AMP kinase in vascular smooth muscle. J Appl Physiol. 2005 Jan;98(1):296-306. WB, IP
Zang M et al., AMP-activated protein kinase is required for the lipid-lowering effect of metformin in insulin-resistant human HepG2 cells. J Biol Chem. 2004 Nov 12;279(46):47898-905. WB