Anti-RPA70 (IHC)

Anti-RPA70 (IHC)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
IHC-00409-T 10 µl (2500 ng) -

2 - 8 business days*

164.00€
IHC-00409 100 µl (25 µg) -

2 - 8 business days*

623.00€
 
Protein function: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds... more
Product information "Anti-RPA70 (IHC)"
Protein function: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism (PubMed:27723720, PubMed:27723717). Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single- stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S- phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105). [The UniProt Consortium]
Keywords: Anti-RPA1, Anti-REPA1
Supplier: Bethyl Laboratories
Supplier-Nr: IHC-00409

Properties

Application: IHC
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Species reactivity: human, mouse (Expected: rabbit, orangutan, monkey, gorilla, chimpanzee, northern white-cheeked gibbon, gray short-tailed opossum, crab-eating macaque)
Immunogen: synthetic peptide. The epitope recognized by IHC-00409 maps to a region between residues 350 and 400 of human replication protein A1, 70kDa using the numbering given in entry NP_002936.1 (GeneID 6117).
Format: Antigen Affinity Purified

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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