Immunogen:synthetic peptide. The epitope recognized by A300-352A maps to a region between residue 400 and 450 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337).
Bemerkungen:E6AP is an E3 ubiquitin ligase. Inheritance of a mutant maternal copy of this gene results in Angelman syndrome (AS) which is characterized by severe mental retardation, seizures, ataxia, and EEG abnormalities. E6AP interacts with the type 16 and 18 human papillomavirus E6 oncoprotein which results in the targeting of p53 for degradation via the ubiquitin-proteasome system. Alternate names for E6AP include ubiquitin-protein ligase E3A, E6AP ubiquitin-protein ligase, oncogenic protein-associated protein E6-AP, human papillomavirus E6-associated protein, renal carcinoma antigen NY-REN-54, UBE3A, E6AP, EPVE6AP, HPVE6A, AS, ANCR, and FLJ26981.
Rabbit anti-E6AP Antibody, Affinity Purified
Detection of Human and Mouse E6AP by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody A300-352A (lot A300-352A-2) used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody A300-351A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Gene and Protein Information
X. laevis, X. tropicalis, Chicken, Turkey, Rabbit, Guinea pig_10141, Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Percomorpha, Siniperca kneri, Teleostei, Thirteen-lined ground squirrel, Nile tilapia, Smegmamorpha, Chinese perch, African elephant, European seabass, Japanese pufferfish, Jarbua terapon, Gray short-tailed opossum, Red drum, Rock bass, White-tufted-ear marmoset, White bass, Three-spined stickleback, Small-eared galago, Zebra finch, Medaka fish, Labroidei, European domestic ferret, Cichlidae, Percoidei, Tasmanian devil, Spotted green pufferfish, Largemouth bass, Japanese sea perch, Barramundi, Flathead mullet, Green anole, Duckbill platypus, Naked mole rat, Northern white-cheeked gibbon, Blue seachub, Crab-eating macaque, Little brown bat less...
Meng L et al., Towards a therapy for Angelman syndrome by targeting a long non-coding RNA. Nature. 2014 Dec 1. IHC-P
Holloway A et al., Resistance to UV-induced apoptosis by ?-HPV5 E6 involves targeting of activated BAK for proteolysis by recruitment of the HERC1 ubiquitin ligase. Int J Cancer. 2014 Nov 19. WB
Pignatelli M et al., Changes in mGlu5 Receptor-Dependent Synaptic Plasticity and Coupling to Homer Proteins in the Hippocampus of Ube3A Hemizygous Mice Modeling Angelman Syndrome. J Neurosci. 2014 Mar 26;34(13):4558-66. WB
Filonova I et al., Activity-dependent changes in MAPK activation in the Angelman Syndrome mouse model. Learn Mem. 2014 Jan 16;21(2):98-104. WB
Meng L et al., Truncation of Ube3a-ATS Unsilences Paternal Ube3a and Ameliorates Behavioral Defects in the Angelman Syndrome Mouse Model. PLoS Genet. 2013 Dec;9(12):e1004039. WB
Condon KH et al., The Angelman Syndrome Protein Ube3a/E6AP Is Required for Golgi Acidification and Surface Protein Sialylation. J Neurosci. 2013 Feb 27;33(9):3799-814. WB, IHC-P
Daily J et al., Spatial and temporal silencing of the human maternal UBE3A gene. Eur J Paediatr Neurol. 2012 Nov;16(6):587-91. WB
Meng L etc., Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a. Hum Mol Genet. 2012 Jul 1;21(13):3001-12.
Daily JL et al., Adeno-associated virus-mediated rescue of the cognitive defects in a mouse model for Angelman syndrome. PLoS One. 2011;6(12):e27221. IHC-P
Ramamoorthy S et al., Isoform-specific degradation of PR-B by E6-AP is critical for normal mammary gland development. Mol Endocrinol. 2010 Nov;24(11):2099-113. WB
Sato M et al., Genomic imprinting of experience-dependent cortical plasticity by the ubiquitin ligase gene Ube3a. Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5611-6. WB, ICC
Reelfs O et al., Thiothymidine plus low-dose UVA kills hyperproliferative human skin cells independently of their human papilloma virus status. Mol Cancer Ther. 2007 Sep;6(9):2487-95. WB
Wu MY et al., Deficiency of Rbbp1/Arid4a and Rbbp1l1/Arid4b alters epigenetic modifications and suppresses an imprinting defect in the PWS/AS domain. Genes Dev. 2006 Oct 15;20(20):2859-70. WB
Jiang YH et al., A mixed epigenetic/genetic model for oligogenic inheritance of autism with a limited role for UBE3A. Am J Med Genet A. 2004 Nov 15;131(1):1-10.